CATALASE ACTIVITY IN INTERACTION WITH IONIZED WATER AND OTHER ANTIOXIDANTS IN BLOOD PLASMA, LIVER, AND KIDNEY OF THE RAT DURING HYPERTHERMIC STRESS

Authors

  • Majlinda Ademi Faculty of Medical Sciences, Study Program of General Medicine, University of Tetovo, Republic of North Macedonia

DOI:

https://doi.org/10.35120/medisij010421a

Keywords:

catalase, ionized water, blood plasma, liver, kidney

Abstract

The similarity between the responses seen after heat stress and those happening in the condition of oxidative stress suggests that heat stress is an environmental element that stimulates the generation of reactive oxygen species (ROS). Alkaline water, also known as ionized or reduced water (ERW), is water that has undergone electrochemical activation and has a pH greater than 7. The ERW also has excellent redox properties and other reducing features. ERW mimics the activity of the antioxidant enzyme, such as catalase (CAT) by scavenging ROS. The aim of this study was to examine the catalase activity in interaction with ERW under hyperthermic stress by including non-enzymatic antioxidants, glutathione, and vitamin C. White laboratory Young female Wistar rats weighing 180–220 g were divided into three groups of 15 for the experiment. Oxidative stress was caused by 41°C acute hyperthermic exposure. The first group is referred to as the control group (CPM), the second group is referred to as the ionized water treatment (TAM), and the third group is referred to as the ionized water treatment with added glutathione and vitamin C (TAD). The treatment period lasted 21 days. The treatment applied respectively to each group during the period of hyperthermic exposure caused a significant difference in CAT activity in blood plasma among the three groups. Liver CAT activity was increased in all three groups. Treatment for 21 days in all three groups led to a decrease in CAT activity in the blood plasma and in the kidneys. Acute hyperthermic exposure on the 21st day in the CPM and TAD groups for blood plasma has a statistically significant difference (p < 0.01). Also, in both the TAM and TAD groups there is a statistically significant difference (p < 0.01) in CAT activity, which is in contrast to the difference in the liver and kidney CAT activity between the remaining compared groups, which was shown to be statistically insignificant.

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Published

2022-12-14

How to Cite

Ademi, M. (2022). CATALASE ACTIVITY IN INTERACTION WITH IONIZED WATER AND OTHER ANTIOXIDANTS IN BLOOD PLASMA, LIVER, AND KIDNEY OF THE RAT DURING HYPERTHERMIC STRESS . MEDIS – International Journal of Medical Sciences and Research, 1(4), 21–25. https://doi.org/10.35120/medisij010421a

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