ANTIBACTERIAL ACTIVITY OF A NOVEL GREEN-SYNTHESIZED NANO-SILVER FLUORIDE AGAINST STREPTOCOCCUS MUTANS: A COMPARATIVE IN VITRO STUDY

Antonia Palankalieva; Iliyan Dobrev; Bogdan Goranov; Ani Belcheva

doi:10.35120/medisij0503113p

Authors

Antonia Palankalieva Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Plovdiv, Bulgaria Author
Iliyan Dobrev Department of Microbiology, University of Food Technologies - Plovdiv, Bulgaria Author
Bogdan Goranov University of Food Technologies - Plovdiv, Bulgaria Author
Ani Belcheva Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Plovdiv, Bulgaria Author

DOI:

https://doi.org/10.35120/medisij0503113p

Keywords:

silver diamine fluoride, nano-silver fluoride, green synthesis, Streptococcus mutans, antibacterial activity

Abstract

Background: Silver diamine fluoride is an effective non-invasive material for caries arrest, but its clinical use is limited due to the black discoloration it causes. Green synthesis of silver nanoparticles has emerged as a promising non-discoloring alternative with antimicrobial potential. Purpose: To evaluate the antibacterial activity of a novel green-synthesized nano-silver fluoride prepared with Camellia sinensis extract and compare its efficacy with silver diamine fluoride and silver diamine fluoride combined with potassium iodide against Streptococcus mutans. Methodology: Antibacterial activity was assessed using the agar well diffusion method. Three concentrations (100%, 10% and 1%) of each material were tested and inhibition zone diameters were measured. The data were analyzed using non-parametric statistical methods with a significance level of p<0.05. Results: Silver diamine fluoride demonstrated the strongest antibacterial activity at all tested concentrations. The green-synthesized nano-silver fluoride exhibited concentration-dependent antimicrobial activity. No statistically significant differences were observed between the highest concentrations of nano-silver fluoride, silver diamine fluoride and silver diamine fluoride combined with potassium iodide. Potassium iodide alone showed no antibacterial effect. Conclusions: The green-synthesized nano-silver fluoride demonstrated significant concentration-dependent antibacterial activity against Streptococcus mutans. Although its inhibition zones were smaller than those of silver diamine fluoride, its activity at higher concentrations suggests promising potential as an alternative silver-based agent for non-operative caries management.

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References

Alvarez-Marín, C. A., Robles-Bermeo, N. L., Hassan Moustafa, W. H., & Medina-Solís, C. E. (2024). Antibacterial effects of silver diamine fluoride with and without potassium iodide against Streptococcus mutans. Contemporary Clinical Dentistry, 15(1), 22-26. https://doi.org/10.4103/ccd.ccd_393_23

Anita, P., Balan, I. N., Ethiraj, S., Madan Kumar, P., & Sivasamy, S. (2015). In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. Journal of Basic and Clinical Pharmacy, 6(1), 35-39. https://doi.org/10.4103/0976-0105.145777

Chahardoli, A., Safaei, M., Mobarakeh, M. S., Fallahnia, N., Fatehi, B., Imani, M. M., & Golshah, A. (2022). Optimum green synthesis of silver nanoparticles with the highest antibacterial activity against Streptococcus mutans biofilm. Journal of Nanomaterials, 2022, Article 6261006. https://doi.org/10.1155/2022/6261006

Costa, R. O., Macedo, P. M., Carvalhal, A., & Bernardes-Engemann, A. R. (2013). Use of potassium iodide in dermatology: Updates on an old drug. Anais Brasileiros de Dermatologia, 88(3), 396-402.

Gao, S. S., Amarquaye, G., Arrow, P., Bansal, K., Bedi, R., Campus, G., Chen, K. J., Chibinski, A. C. R., Chinzorig, T., Crystal, Y. O., Duangthip, D., Ferri, M. L., Folayan, M. O., Garidkhuu, A., Hamama, H. H., Jirarattanasopha, V., Kemoli, A., Leal, S. C., Leelataweewud, P., … Chu, C. H. (2021). Global oral health policies and guidelines: Using silver diamine fluoride for caries control. Frontiers in Oral Health, 2, 685557. https://doi.org/10.3389/froh.2021.685557

Greenwall-Cohen, J., Greenwall, L., & Barry, S. (2020). Silver diamine fluoride—An overview of the literature and current clinical techniques. British Dental Journal, 228(11), 831-838. https://doi.org/10.1038/s41415-020-1641-4

Karaduran, B., Çelik, S., Üçüncü, M. Y., Topçuoğlu, N., Gök, M. K., & Koruyucu, M. (2024). Antibacterial effects of silver diamine fluoride, potassium iodide and nanosilver fluoride on dual-species biofilm. Journal of Dentistry, 147, 105097. https://doi.org/10.1016/j.jdent.2024.105097

Kumar, A., Majumdar, R. S., & Dhewa, T. (2018). In vitro efficacy of biosynthesized AgNPs against Streptococcus mutans causing dental plaque formation. Journal of Scientific & Industrial Research, 77(4), 225-228.

Lansdown, A. B. G. (2006). Silver in health care: Antimicrobial effects and safety in use. Current Problems in Dermatology, 33, 17-34. https://doi.org/10.1159/000093928

Liao, C., Li, Y., & Tjong, S. C. (2019). Bactericidal and cytotoxic properties of silver nanoparticles. International Journal of Molecular Sciences, 20(2), 449. https://doi.org/10.3390/ijms20020449

Nguyen, V., Neill, C., Felsenfeld, J., & Primus, C. (2017). Potassium iodide: The solution to silver diamine fluoride discoloration? Advances in Dentistry & Oral Health, 5(1), 555565. https://doi.org/10.19080/ADOH.2017.05.555565

Palankalieva, A., Katsarov, P., & Belcheva, A. (2025). Green-synthesized nano-silver fluoride for remineralization of enamel lesions in primary teeth: A comparative in vitro study with SDF and SDF/KI. Dentistry Journal, 13(7), 331. https://doi.org/10.3390/dj13070331

Pérez-Díaz, M. A., Boegli, L., James, G., Velasquillo, C., Sánchez-Sánchez, R., Martínez-Martínez, R.-E., Martínez-Castañón, G. A., & Martinez-Gutierrez, F. (2015). Silver nanoparticles with antimicrobial activities against Streptococcus mutans and their cytotoxic effect. Materials Science and Engineering: C, 55, 360-366. https://doi.org/10.1016/j.msec.2015.05.036

Sayed, M., Matsui, N., Hiraishi, N., Nikaido, T., Burrow, M. F., & Tagami, J. (2018). Effect of glutathione bio-molecule on tooth discoloration associated with silver diammine fluoride. International Journal of Molecular Sciences, 19(5), 1322. https://doi.org/10.3390/ijms19051322

Sharma, P., Dhawan, P., Rajpal, S. K., & Sharma, R. (2023). A comparison of antimicrobial efficacy of silver-based preventive restorations (silver nitrate, silver diamine fluoride, and silver nanoparticles) against Streptococcus mutans monospecies biofilm model. International Journal of Clinical Pediatric Dentistry, 16(Suppl. 1), S13-S19. https://doi.org/10.5005/jp-journals-10005-2575

Sissons, C. H., Cutress, T. W., Hoffman, M. P., & Wakefield, J. S. (1991). A multi-station dental plaque microcosm (artificial mouth) for the study of plaque growth, metabolism, pH, and mineralization. Journal of Dental Research, 70(11), 1409-1416.

Soleimani, B., Goli, H., Naranjian, M., Mousavi, S. J., & Nahvi, A. (2021). Comparison of antimicrobial activity of fluoride varnishes against Streptococcus mutans and Lactobacillus acidophilus: An in vitro study. Iranian Journal of Pediatrics, 31(3), e111422. https://doi.org/10.5812/ijp.111422

Wu, L., Gareiss, S. K., Morrow, B. R., Babu, J. P., Hottel, T., Garcia-Godoy, F., Li, F., & Hong, L. (2019). Antibacterial properties of silver-loaded gelatin sponges prepared with silver diamine fluoride. American Journal of Dentistry, 32(6), 276-280.

Yin, I. X., Zhang, J., Zhao, I. S., Mei, M. L., Li, Q., & Chu, C. H. (2020). The antibacterial mechanism of silver nanoparticles and its application in dentistry. International Journal of Nanomedicine, 15, 2555-2562. https://doi.org/10.2147/IJN.S246764