THE INFLUENCE OF THE MODE OF DELIVERY ON FETAL COLONISATION – VAGINAL DELIVERY VS CESARION SECTION
DOI:
https://doi.org/10.35120/medisij040221mKeywords:
placental microbiome, amniotic fluid, vernix, delivery, real time polymerase chain reactionAbstract
The last decade, the existence of placental microbiota is debated and there is no consensus regarding the existence of a placental microbiome in healthy full-term pregnancies(Aagaard et al., 2014; Perez-Muñoz et al., 2017). There are still opposite meanings about the fetal colonization, weather it begins in utero, or the first interaction with bacteria is after delivery. In this study, we aimed to investigate the fetal environment to determine whether there is an intrauterine microbiome or if colonization begins at birth. A cross-sectional study was performed, comparing samples from placental tissue, amniotic fluid, and vernixcaseosa from two groups of full-term births: women not in labor undergoing elective cesarean deliveries and women with vaginal deliveries. Women with complications, infections, and preterm deliveries were excluded. Real-time PCR (polymerase chain reaction) was used for the detection of bacteria. A ready-made amplification kit – Vaginitis Screening Assay Allplex™ (Seegene Inc., n.d.) – was used for molecular testing, and results were interpreted using the Seegene Viewer. Our results showed no evidence of fetal colonization in utero; rather, the mode of delivery plays a significant role in the colonization of the newborn and establishment of the first microbiome (Dominguez-Bello et al., 2010; Gritz&Bhandari, 2015).
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Aagaard, K., Ma, J., Antony, K. M., Ganu, R., Petrosino, J., &Versalovic, J. (2014). The placenta harbors a
unique microbiome. Science TranslationalMedicine, 6(237), 237ra65. https://doi.org/10.1126/scitranslmed.3008599 DOI: https://doi.org/10.1126/scitranslmed.3008599
Azad, M. B., Konya, T., Persaud, R. R., Guttman, D. S., Chari, R. S., Field, C. J., ... &Kozyrskyj, A. L. (2016). Impact of maternal intrapartum antibiotics, method of birth and breastfeeding on gut microbiota during the first year of life: a prospective cohort study. BJOG: An International Journal of Obstetrics &Gynaecology, 123(6), 983–993. https://doi.org/10.1111/1471-0528.13601 DOI: https://doi.org/10.1111/1471-0528.13601
Dominguez-Bello, M. G., Costello, E. K., Contreras, M., Magris, M., Hidalgo, G., Fierer, N., & Knight, R. (2010). Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proceedings of the National Academy of Sciences, 107(26), 11971–11975. https://doi.org/10.1073/pnas.1002601107 DOI: https://doi.org/10.1073/pnas.1002601107
Gritz, E. C., &Bhandari, V. (2015). The human neonatal gut microbiome: A brief review. Frontiers in Pediatrics, 3, 17. https://doi.org/10.3389/fped.2015.00017 DOI: https://doi.org/10.3389/fped.2015.00017
MacIntyre, D. A., Chandiramani, M., Lee, Y. S., Kindinger, L., Smith, A., Angelopoulos, N., Lehne, B., Arulkumaran, S., Brown, R., Teoh, T. G., Holmes, E., and Bennett, P. R. (2021). “The Vaginal Microbiome during Pregnancy and the Postpartum Period in a European Population.” Scientific Reports 11(1):12101. https://doi.org/10.1038/s41598-021-91524-w.
Mitchell, C. M., Mazzoni, C., Hogstrom, L. J., Bryant, A., Bergerat, A., Cher, A. & Dominguez-Bello, M. G. (2020). Delivery mode affects stability of early infant gut microbiota. Cell Reports Medicine, 1(6), 100156. https://doi.org/10.1016/j.xcrm.2020.100156 DOI: https://doi.org/10.1016/j.xcrm.2020.100156
Park, H. J., Lee, S. W., Park, H. D., Kim, B. I., and Choi, J. Y. (2023). “Evidence for In Utero Colonization by Microbial DNA in Neonatal Gastric Fluid and Meconium.” Microorganisms 11(2) :292 https://doi.org/10.3390/microorganisms11020292. DOI: https://doi.org/10.3390/microorganisms11020292
Perez-Muñoz, M. E., Arrieta, M. C., Ramer-Tait, A. E., & Walter, J. (2017). A critical assessment of the “sterile womb” and “in utero colonization” hypotheses: Implications for research on the pioneer infant microbiome. Microbiome, 5(1), 48. https://doi.org/10.1186/s40168-017-0268-4 DOI: https://doi.org/10.1186/s40168-017-0268-4
Seegene Inc. (n.d.). Allplex™ Vaginitis Screening Assay [Product information]. https://www.seegene.com/
Shao, Y., S. C. Forester, E. Vervier, A. Strang, N. Simpson, N. Kumar, M. D. Stares, A. Rodger, P. Brocklehurst, and E. Holmes. (2019). “Stunted Microbiota and Opportunistic Pathogen Colonization in Caesarean-Section Birth.” Nature 574(7778):117–121. https://doi.org/10.1038/s41586-019-1560-1. DOI: https://doi.org/10.1038/s41586-019-1560-1
Stewart, C. J., N. J. Ajami, J. L. O’Brien, D. S. Hutchinson, D. P. Smith, M. C. Wong, M. C. Ross, R. E. Lloyd, H. Doddapaneni, G. A. Metcalf, and E. Sodergren. (2018). “Temporal Development of the Gut Microbiome in Early Childhood from the TEDDY Study.” Nature 562(7728):583–588. https://doi.org/10.1038/s41586--18-0617-x. DOI: https://doi.org/10.1038/s41586-018-0617-x
Yu, L., Y. Guo, and J. L. Wu. (2024). “Influence of Mode of Delivery on Infant Gut Microbiota Composition: A Pilot Study.”Journal of Obstetrics and Gynecology 44(1):2368829. https://doi.org/10.1080/01443615.2024.2368829. DOI: https://doi.org/10.1080/01443615.2024.2368829
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